. 2013 May 9;8(5):e63647. doi: 10.1371/journal.pone.0063647

Table 3. Expression of msrA-lacZ and msrB-lacZ fusions in wild type, ryhB deletion or fur deletion strains grown to exponential phase (O.D.₆₀₀≈0.4) in LB rich medium either in the presence or in the absence of iron chelator (2,2′dip, 250 µM).

Type of fusion	Strains	LB	LB +2,2′dip	Fold expression ±2,2′dip
Transcriptional	msrB’-lacZ (JB43)	1655±35	1502±30	0.9x
Translational	msrB’-‘lacZ (JB44)	680±20	190±8	0.3x
Translational	msrB’-‘lacZ ryhB ::cat (JB50)	688±16	702±8	1.0x
Translational	msrB’-‘lacZ fur ::kan (JB46)	181±6	172±9	1.0x
Transcriptional	msrA’-lacZ (JB55)	105±32	148±35	1.4x
Translational	msrA’-‘lacZ (JB56)	58±14	79±4	1.4x
Translational	msrA’-‘lacZ ryhB ::cat (JB64)	53±6	93±13	1.7x
Translational	msrA’-‘lacZ fur ::kan (JB61)	53±5	60±10	1.1x
Translational	msrA’-‘lacZ ryhB ::cat fur ::kan (YD100)	34±5	47±14	1.4x

The level of β-galactosidase expressed from the fusions is given in Miller units. Each value is the average of four independent experiments with three measurements each. Fold expression in iron starvation is indicated. Standard error is indicated.

Table 3. Expression of msrA-lacZ and msrB-lacZ fusions in wild type, ryhB deletion or fur deletion strains grown to exponential phase (O.D.600≈0.4) in LB rich medium either in the presence or in the absence of iron chelator (2,2′dip, 250 µM).

Table 3. Expression of msrA-lacZ and msrB-lacZ fusions in wild type, ryhB deletion or fur deletion strains grown to exponential phase (O.D.₆₀₀≈0.4) in LB rich medium either in the presence or in the absence of iron chelator (2,2′dip, 250 µM).