Skip to main content
. 2013 May 9;8(5):e63845. doi: 10.1371/journal.pone.0063845

Figure 1. CURN inhibits TNF-α-induced in ICAM-1 gene and protein levels in A549 cells.

Figure 1

(A) A549 cells were incubated with CURN (0–100 µM) for 24 h, and then the cell viability was determined by MTT assay. (B, E) A549 cells were pretreated with CURN or CURH (5, 10, or 50 µM) or PVP for 1 h, and then incubated with 30 ng/ml TNF-α for 24 h. The levels of ICAM-1 protein were determined by Western blot. (C) A549 cells were pretreated with 50 µM CURN, CURH or DPI (10 µM) for 1 h, and then incubated with 30 ng/ml TNF-α for 6 h. The expression of ICAM-1 mRNA was determined by RT-PCR. (D) Cells were transiently transfected with ICAM-1-luc reporter gene, pretreated with CURN or CURH for 1 h, and then incubated with TNF-α for 6 h. The ICAM-1 promoter activity was determined in the cell lysates. (F) A549 cells were pretreated with 50 µM CURN, CURH, DPI (10 µM) or Tanshinone II A(10 µM) for 1 h, and then incubated with 30 ng/ml TNF-α for 24 h. The U937 cells adherence was measured. The data are expressed as mean±S.E.M. of three independent experiments. *P<0.05; ** P<0.01, significant with respect to the basal level.