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. 2013 May 9;8(5):e64202. doi: 10.1371/journal.pone.0064202

Figure 1. LGP2 enhances IFN induction in response to poly(I:C).

Figure 1

(A–C) HEK293 cells were transfected with a reporter plasmid containing the luciferase gene under the control of the IFN-β promoter, a plasmid constitutively expressing β-galactosidase as a transfection control, and (A) 0.4 ng plasmids expressing mda-5 or RIG-I, (B) 100 ng plasmid expressing LGP2, or (C) 0–160 ng plasmid expressing LGP2. Total amounts of DNA were kept constant by supplementing with the empty vector pEFpl2. 24 hours after transfection cells were further transfected with the indicated amounts of poly(I:C) for 16 hours. Cell lysates were analysed for luciferase and β-galactosidase activity, and relative expression levels calculated. The effect of LGP2 on induction by poly(I:C) is statistically significant (p<0.01).