Figure 3. LGP2 co-operates with mda-5 but not RIG-I to promote poly(I:C) signaling.

HEK293 cells were transfected with the IFN-β reporter plasmid, the β-galactosidase expression plasmid, and combinations of plasmids expressing mda-5 (0.4 ng), RIG-I (0.4 ng) or LGP2 (2 ng). 24 hours after transfection cells were further transfected with increasing amounts of poly(I:C) for 16 hours. Cell lysates were analysed for luciferase and β-galactosidase activity, and relative expression levels calculated.