Figure 5. Analysis of the regions of LGP2 required for stimulation of poly(I:C) signaling.

(A) Schematic diagram of LGP2 mutants. (B–E) HEK293 cells or LGP2 knockdown cells were transfected with the IFN-β reporter plasmid, the β-galactosidase expression plasmid, and plasmids expressing mda-5, RIG-I, LGP2, or various mutants of LGP2. 24 hours after transfection cells were further transfected with poly(I:C) for 16 hours where indicated. Cell lysates were analysed for luciferase and β-galactosidase activity, and relative expression levels calculated. The average of three independent experiments is shown. (F) HEK293 cells were transfected with plasmids expressing Flag-tagged mutants of LGP2. Lysates of transfected cells were subjected to western blotting using an anti-Flag antibody to confirm expression.