Skip to main content
. 2013 May 9;8(5):e63175. doi: 10.1371/journal.pone.0063175

Figure 1. Nucleosome positioning is progressive during early embryonic development.

Figure 1

(A–D) Average nucleosome density for 37 zebrafish hox promoters was calculated as the log2 ratio of MNase digested to randomly fragmented genomic DNA for positions −600 to +600 relative to the TSS (TSS is set as 0 on X-axis) at 2 hpf (A), 4 hpf (B), 6hpf (C) and 9hpf (D). Detectable nucleosome peaks are numbered in panels C (at positions +60, +260 and +480, separated by 200 bp and 220 bp respectively) and D (at positions −450, −290, −170, +155, and +250 bp, separated by 150 bp, 120 bp, 290 bp, and 130 bp respectively). Arrow in panel D indicates a nucleosome depleted region (NDR) formed between the −1 and +1 nucleosomes.