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. 2013 May 9;8(5):e63175. doi: 10.1371/journal.pone.0063175

Figure 2. Nucleosome organization differs between expressed and non-expressed promoters.

Figure 2

(A–L) Average nucleosome density was calculated as in figure 1 for expressed (red line in panels A, B, D, E, G, H, J, K) and non-expressed (blue lines in panels A, C, D, F, G, I, J, L) promoters at 2 hpf (A–C), 4 hpf (D–F), 6 hpf (G–I) and 9 hpf (J−L). Nucleosome densities at expressed and non-expressed promoters were compared using a Wilcoxon Ranked Sum test and statistically significant differences (p<0.05) are illustrated in green on the horizontal line in panels A, D, G, J. Arrows in D, G, and J indicate the −1 nucleosome. (M, N) Overlay of profiles for expressed (M) and non-expressed (N) promoters at all time points. Arrow in M indicates region where 2 hpf time point (red line) has greater nucleosome density than later time points. (O) Change in occupancy of the −1 nucleosome was calculated as a ratio of density at the −1 nucleosome to density at the +1 nucleosome for expressed (red bars) and non-expressed (blue bars) promoters at 4 hpf, 6 hpf and 9 hpf.