Figure 3. DEAB treatment blocks hox transcription.
(A–F) Zebrafish embryos were left untreated (blue bars) or treated with 5 uM (green bars), 10 uM (red bars) or 20 uM (purple bars) DEAB and harvested at 9 hpf. Transcript levels for hoxb1a (A), hoxb7a (B), hoxb5b (C), hoxb6b (D), hoxc8a (E) and hoxc9a (F) were determined by quantitative RT-PCR and normalized to β-actin. Error bars indicate standard deviations of 3 technical replicates.