Skip to main content
. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Plasmid. 2013 Jan 23;69(3):211–222. doi: 10.1016/j.plasmid.2013.01.002

Figure 4.

Figure 4

Deletion of the qseB gene in A. actinomycetemcomitans 652. (A). Schematic representation of the ygiW-qseB-qseC locus. The transcriptional orientation of genes is indicated by open arrows. Thin arrows represent primers used for PCR confirmation of the deletion of qseB. (B). PCR products obtained with the A. actinomycetemcomitans ΔqseB mutant strains. Deletion of the qseB gene was confirmed by PCR amplification of a 929-bp fragment with primer set MDJR-63F and MDJR-61R in ten randomly selected SucR and SpS colonies obtained with pDJR37 suicide vector as described in Material and Methods. The amplification profile used was: 94°C for 2 min for 1 cycle and then 94°C for 30 s, 58°C for 30 s, and 68°C for 2 min for 35 cycles. MWM, molecular weight marker.