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Shear stress-induced changes in RhoA activity are dependent on cytoskeleton and intracellular tension. (A) 5 dyn/cm2 (n > 5 cells). (B) 20 dyn/cm2 (n > 5 cells). The cells were transfected with RhoA biosensor and then treated with drugs. Actin filament disruption (CytoD and LatA), inhibiton of myosin light chain kinase (ML-7), or inhibition of myosin II (Bleb) prevented shear stress-induced RhoA activity.
