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Cryo-TEM images of liposomes formed using a lipid solution containing (a) 0% and (b) 10% PEG-lipid. Imaging results confirm the formation of unilamellar vesicles absent micelles or aggregates in both cases. Small ice crystals or artifacts that commonly form during vitrification, sample transfer, or elevation of specimen temperature due to the electron beam during cryo-TEM imaging seen in several images [28,29] do not reflect the presence of lipid aggregates.
