Fig. 2.

Cardiac inflammatory responses and DNA binding activities of redox-regulated transcription factors in the left ventricle of Dahl salt-sensitive (DS) rats. Aa–d Interstitial collagen deposition in the left ventricle as demonstrated by Azan-Mallory staining. e–l Immunostaining of LV tissue for macrophages (e–h) and T lymphocytes (i–l) of DS rats fed a low-salt diet (0.3 % NaCl) or a high-salt diet and treated either with vehicle (8 % NaCl) or with fenofibrate at doses of 30 mg/kg per day [8 % NaCl + peroxisome proliferator-activated receptor-α (PPARα) Act (low)] or 50 mg/kg per day [8 % NaCl + PPARα Act (high)]. Arrows Infiltrated immunoreactive cells. B Representative electrophoretic mobility-shift assays for the binding activities of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). Lanes: a Free extracts, b low-salt diet group, c high-salt diet group, d high-salt diet + PPARα Act (low) group, e high-salt diet + PPARα Act (high) group, f cold probe. C Quantitative data expressed relative to the corresponding value of the low-salt diet group. Results are presented as the mean ± SEM of 4 animals in each experiment. *P < 0.05 vs. rats fed the low-salt diet, ^†P < 0.05 vs. rats fed the high-salt diet and treated with vehicle