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a Symptom of yellowing, puckering and vein thickening in ash gourd leaf. b Detection of begomovirus through PCR. Lane 1–7: symptomatic sample yielded a 1.2 kb amplicon which was not present in asymptomatic samples. c RCA product digested with BglI (lane 1), EcoRV (lane 2), PstI (lane 3) and XbaI. M 1 kb molecular weight marker, AS asymptomatic sample. (Color figure online)
