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. 2013 Apr 15;27(8):887–899. doi: 10.1101/gad.215244.113

Figure 3.

Figure 3.

The NAD+/NADH ratio increases with increasing iron concentration. (A) The NAD+/NADH ratio for the wild strain 3610 grown for 48 h on solid biofilm-inducing medium containing the indicated levels of iron. Pairs of colonies were harvested and extracted under either acidic or basic conditions for measurements of NAD+ and NADH, respectively. The NAD+/NADH ratios are the average for six colonies (representing three acidic and three basic extractions) for each iron concentration. (B) Expression of Pldh-lacZ in a wild strain carrying the fusion (AE276). Cells were grown on solid biofilm-inducing medium containing the indicated concentration of iron for 48 h. After 48 h, colonies were harvested, and Pldh activity was measured. The results are the average for three colonies for each iron concentration.