Figure 6.
KinB function depends on transmembrane segments 1 and 2. (A) The structure of the KinB sensor kinase. (B) Colony morphologies for the wild-type strain and the indicated mutants (obtained as described in the Supplemental Material) after 72 h of growth on biofilm-inducing medium containing the indicated iron levels. (C) Colony morphologies for a kinA kinB double mutant containing a wild-type copy of kinB at amyE or the indicated mutant alleles (obtained as described in the Supplemental Material). Colonies were grown for 72 h on medium containing 250 μM iron. (D) Levels of PsinI-lacZ expression in a kinA kinB double mutant complemented with either wild-type kinB or the indicated kinB mutant alleles.