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. 2004 Feb 3;5:11. doi: 10.1186/1471-2164-5-11

Table 4.

Different approaches used for identification of amplified genes in (IMR-32) neuroblastoma cell lines

Methodology Publication MYCN DDX1 NAG NSE1 g10d12 DNMT3A TEM8 g4d5 MEIS1 g2h10
cloning after MYC Southern blot hybridization Schwab et al. (1983) [18] X
subtractive cDNA cloning and Southern blot Manohar et al. (1995) [39] X
differential cDNA library screening Godbout and Squire (1993); Squire et al. (1995) [19,40] X
2D separation of genomic restriction fragments Wimmer et al. (1999) [15] X
screening PAC/BAC libraries after microdissection Jones et al. (2000) [14] X
serendipitous cloning after multiprobe Southern blot Spieker et al. (2001) [13] X
CGH on cDNA microarrays Beheshti et al. (2003) [17] X X X X X
combined subtractive cDNA cloning and array CGH this study X X X X X X X X X