Figure 6.

Dual-target: data before normalisation For all dual-target hybridisations, these box-plots show the signal distributions across all genes for each individual dye-label sample. The non-hybridised "blank" dye-label channel has also been scanned and processed, in order to assess the effect of signal bleeding from one dye-label to another. The only truly blank scan is Cy5 for the Cy3/Alexa594 co-hybridisations. Co-hybridisations of the standard Cy5/Cy3 and new Cy5/Alexa594 dye-labels leads to small, but detectable levels of signal in the blank scan. Regarding spread of data, the dual-target hybridisation are identical to the results of the triple-target hybridisations, with a wider distribution and lower average of signal for Cy5.