Table 1.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Relevant genotype or phenotype | Reference or source |
| Strains | ||
| B. subtilis 168 | wild type | BGSCa, 1A1 |
| E. coli | ||
| RB791 | F' [lacIq L8] hsdR+ hsdM | [32] |
| UE26 | F- ptsG2 ptsM1 glk7 rpsL150 | [33] |
| Plasmids | ||
| pMD496 | pQE9 derivative carrying glcK fused in frame to the His tag-coding region | [6] |
| pLM-GlcK [D22K] | pMD496 derivative carrying glcKD10K | This study |
| pLM-GlcK [C178A] | pMD496 derivative carrying glcKC166A | This study |
| pLM-GlcK [C187A] | pMD496 derivative carrying glcKC175A | This study |
| pLM-GlcK [C189A] | pMD496 derivative carrying glcKC177A | This study |
| pLM-GlcK [C194A] | pMD496 derivative carrying glcKC182A | This study |
| pLM-GlcK [C294A] | pMD496 derivative carrying glcKC282A | This study |
| pLM-GlcK [C333A] | pMD496 derivative carrying glcKC321A | This study |
a BGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus, Ohio