Table 2.

Sets of primers used for site-directed mutagenesis in this study

Mutation	Primer setsa
D10K	5' tgcgggcattaagctgggaggaacgacgat 3'
	5' aaccatatctcgtccatggatccgtgatgg 3'
C166A	5' ggccatattgccagcatccctgaaggcgga 3'
	5' gatttctccgccggcgccatttataccatg 3'
C175A	5' gcgcccgccaactgcggcaaaacgggctgt 3'
	5' tccgccttcagggatgctgcaaatatggcc 3'
C177A	5' tgcaacgccggcaaaacgggctgtatcgaa 3'
	5' gggcgctccgccttcagggatgctgcaaat 3'
C182A	5' ggcaaaacgggcgctatcgaaacaattgcg 3'
	5' gcagttgcagggcgctccgccttcagggat 3'
C282A	5' cgcaaagccgcgtttccgcgggcagcccaa 3'
	5' gaatgttttctcgacttttgatctcagcag 3'
C321A	5' catcaaaatgcttaaaattgtgtaaatgaa 3'
	5' tttcagccattcatttttagcgatccaagc 3'

^a Underlined is mutated nucleotide.