Figure 2. SIRT4 is induced by DNA damage stimuli.
(A) Relative mRNA expression levels of indicated sirtuins in HEK293T cells treated with 14 μM CPT or 25 μM ETS for 15 hr were measured by qRT-PCR (n = 4). β-ACTIN was used as an endogenous control for qRT-PCR.
(B) Relative Sirt4 mRNA levels in primary MEFs at 12 hr after treatment with CPT (14 μM), ETS (25 μM), IR (5 Gy) or UV (30 J/m2) were measured by qRT-PCR (n = 3–4). β-Actin was used as an endogenous control for qRT-PCR.
(C) SIRT4 protein in whole cell lysates from HEK293T cells treated with CPT (14 μM) or ETS (25 μM) for 15 hr was detected by immunoblotting with anti-human SIRT4. β-ACTIN serves as a loading control.
(D) SIRT4 protein in transformed WT and SIRT4 KO MEFs treated CPT (14 μM) for the indicated times. β-Actin serves as a loading control.
Data are means ±SEM. *p < 0.05, **p < 0.005, ***p < 0.0001. All experiments were performed at least three times in duplicate.
See also Figure S2.