Fig. 5.

A substantial fraction of tumor cell secretomes comes from non-classical secretion. Bar plots showing SpC quantification of selected proteins secreted by MCF7 cells when treated with Brefeldin A compared with the non-treated cells, and from exosomes isolated from the conditioned media of the same cells under identical experimental conditions. The top panel shows secreted proteins enriched in exosomes, the middle panel shows classically secreted proteins. The last panel shows secreted proteins unconventionally secreted but not enriched in the exosomes. The standard deviation was made from three biological replicates, each of them containing three technical replicates.