Fig. 2.
Distribution profiles of unlabeled-to-labeled protein ratios. Gastrocnemius muscle was harvested from SILAC-labeled C57BL/6 mice (F2 generation) and from age-matched unlabeled mdx-52 and unlabeled C57BL/6 mice. SILAC ratios obtained for unlabeled C57BL/6 versus labeled C57BL/6 (blue) and unlabeled mdx versus labeled C57BL/6 (red) were transformed to log values and plotted. An overlay for these plots shows the distribution profiles. Ratios of unlabeled to labeled peptide pairs were obtained using IP2 software. The overall mean ± S.D. was 1.52 ± 1.3 for unlabeled mdx versus labeled C57BL/6 and 0.96 ± 0.5 for the unlabeled versus labeled C57BL/6 mice.
