Fig. 4.

Up-regulation of actin cytoskeletal and integrin-linked kinase (ILK) pathways in dystrophic muscle. A, gastrocnemius muscle lysates from C57BL/6 and mdx-52 mice (n = 3/group) were obtained, and homogenates were immunoblotted with antibodies against vimentin, ILK, desmin, and annexin 2. Equal amounts of the protein were electrophoresed in each gel lane, and representative immunoblots are shown. β-actin was used as a loading control. The ratios of respective proteins to β-actin were calculated via densitometric analysis using Quantity One software, and the data are represented as means ± S.E. B–E, quantifications for the respective proteins (*p < 0.05).