Fig. 1.

Identification of Clp1 interacting proteins. A, Schematic of Clp1's domain structure. The catalytic domain is shown in dark green. The PTP motif (residues 281–294), a signature of phosphatases, contains the catalytic site and is highlighted in blue. In the Clp1 substrate trapping mutant, the catalytic site cysteine 286 is replaced by serine. The regulatory domain is shown in lime green. B, Workflow used to identify Clp1-interacting proteins. C, Pie chart showing the different GO categories of all Clp1-interacting proteins (supplemental Table S2). The number of Clp1 interactors with each GO annotation are in indicated in parentheses.