Fig. 4.

Co-immunoprecipitation analysis of potential Clp1 substrates. (A–H) Prior to immunoprecipitation, cells producing the indicated tagged proteins or appropriate control strains were either grown asynchronously, blocked in prometaphase (denoted B) using the cold sensitive β-tubulin mutant, nda3-KM311, or released (denoted R) from the arrest for 30 min., allowing cells to progress into anaphase when Clp1 is most active. Immunoprecipitates were then immunoblotted to detect associations with Clp1 and/or Clp1-C286S.