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. 2013 Jan 7;12(5):1074–1086. doi: 10.1074/mcp.M112.025924

Fig. 6.

Fig. 6.

Role of Cdc11 dephosphorylation by Clp1. A, Cdc11-GFP proteins were immunoprecipitated from the indicated strains and their SDS-PAGE mobilities were determined by immunoblotting with anti-Cdc11 serum. All retarded gel migration of Cdc11 is because of phosphorylation (57, 59). B, The indicated strains were grown at 36 °C in YE, fixed and stained with DAPI and methyl blue to visualize nuclei and cell wall, respectively (left panel). Bar, 5 μm. The number of multiseptated cells was determined and plotted (right panel). dd, double delete and error bars represent standard deviations of the mean. C, The indicated strains were grown to mid-log phase at 25 °C in YE, spotted in 10-fold serial dilutions on YE plates, and incubated at the indicated temperatures.