Table I. Putative E2F2-interacting proteins identified by LC-MS/MS. Uniprot Accession number, Entry name, a brief description, and the subcellular localization of each protein as well as if they are involved in gene expression regulation is indicated. Mascot score, number of identified peptides and sequence coverage shown correspond to the best identification.
| Accesion number | Entry name | Description | Subcellular localizationa | Gene expression regulationa | Mascot score | Peptides | Seq Cov % | |
|---|---|---|---|---|---|---|---|---|
| Conclusive proteins | P05141 | ADT2_HUMAN | ADP/ATP translocase 2 | Mitochondrion inner membrane | 51 | 1 | 9 | |
| P08758 | ANXA5_HUMAN | Annexin A5 | Cytoplasm | 108 | 2 | 9 | ||
| P25705 | ATPA_HUMAN | ATP synthase subunit alpha, mitochondrial | Cell membrane | 76 | 1 | 3 | ||
| Q00610 | CLH1_HUMAN | Clathrin heavy chain 1 | Cytoplasmic vesicle membrane | 137 | 3 | 2 | ||
| Q05639 | EIF3B_HUMAN | Eukaryotic translation initiation factor 3 subunit B | Cytosol | 178 | 4 | 14 | ||
| Q96IT0 | FAS_HUMAN | Fatty acid synthase | Cytoplasm | 238 | 5 | 3 | ||
| P31943 | HNRH1_HUMAN | Heterogeneous nuclear ribonucleoprotein H | Nucleoplasm | 43 | 1 | 3 | ||
| Q14240 | IF4A2_HUMAN | Eukaryotic initiation factor 4A-II | Cytosol | 79 | 1 | 3 | ||
| Q14974 | IMB1_HUMAN | Importin subunit beta-1 | Cytosol; Nuclear membrane; Nucleoplasm | 56 | 2 | 6 | ||
| P12268 | IMDH2_HUMAN | Inosine-5′-monophosphate dehydrogenase 2 | Cytosol; Nucleus | 64 | 1 | 8 | ||
| Q15233 | NONO_HUMAN | Non-POU domain-containing octamer-binding protein | Nucleus | Yes | 88 | 2 | 7 | |
| Q9UNM6 | PSD13_HUMAN | 26S proteasome non-ATPase regulatory subunit 13 | Cytosol; Nucleoplasm | 75 | 1 | 3 | ||
| P62906 | RL10A_HUMAN | 60S ribosomal protein L10a | Cytosol | 79 | 1 | 1 | ||
| P35268 | RL22_HUMAN | 60S ribosomal protein L22 | Cytosol | 47 | 1 | 10 | ||
| P47914 | RL29_HUMAN | 60S ribosomal protein L29 | Cytosol | 49 | 1 | 9 | ||
| Q99729 | ROAA_HUMAN | Heterogeneous nuclear ribonucleoprotein A/B | Nucleus; Cytoplasm | Yes | 46 | 2 | 6 | |
| P09661 | RU2A_HUMAN | U2 small nuclear ribonucleoprotein A' | Nucleus | Yes | 77 | 1 | 13 | |
| Q01130 | SRSF2_HUMAN | Serine/arginine-rich splicing factor 2 | Nucleus; nucleoplasm; Nucleus speckle | Yes | 53 | 1 | 7 | |
| Q9H853 | TB4AB_HUMAN | Putative tubulin-like protein alpha-4B | Cytoplasm | 93 | 2 | 10 | ||
| Q86V81 | THOC4_HUMAN | THO complex subunit 4 | Nucleus; Nucleus speckle; Cytoplasm | Yes | 80 | 1 | 10 | |
| Indistinguishalbe proteins | P62736 | ACTA_HUMAN | Actin, aortic smooth muscle | Cytoplasm; cytoskeleton | 215 | 4 | 14 | |
| P68032 | ACTC_HUMAN | Actin, alpha cardiac muscle 1 | ||||||
| P63267 | ACTH_HUMAN | Actin, gamma-enteric smooth muscle | ||||||
| P68133 | ACTS_HUMAN | Actin, alpha skeletal muscle | ||||||
| Q8NHW5 | RLA0L_HUMAN | 60S acidic ribosomal protein P0-like | Cytosol | 52 | 1 | 7 | ||
| P05388 | RLA0_HUMAN | 60S acidic ribosomal protein P0 |
a Information on subcellular localization and involvement in gene expression regulation was obtained from Uniprot.