Fig. 1.

One-dimensional SDS-PAGE fractionation of sporozoite proteins. The one-dimensional SDS-PAGE separation of a P. yoelii salivary gland sporozoite whole cell lysate (flanked on both sides by a molecular weight (MW) marker) is shown with a bar graph representing the total number of high-quality (false positive error rate < 1.0%) peptide spectrum matches found from a single injection of each fraction after in-gel tryptic digest. Bovine serum albumin (BSA) from the purification protocol can be seen as the large band in fractions 9 and 10. In every other fraction, P. yoelii proteins were the major component.