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PSI monomer band from WT and ΔLepB1 separated into subunits by SDS-PAGE. PSI monomer bands were solubilized as described for the whole BN gel lane and ran side by side on 14% SDS-PAGE with 6 m urea. The area of the low molecular subunits was enlarged in the right part, showing the difference in migration between PsaF in WT and ΔLepB1. The band in WT (3a) and ΔLepB1 (3b) was excised for LC-MS/MS analysis.
