Table I. The ratio of expression LepB1 mutant/WT for protein complexes/proteins involved/related to photosynthesis and respiration. In the first iTRAQ analyses WT was labeled with iTRAQ reagent 114 and LepB1 mutant with 115, 116, and 117. In the second iTRAQ analyses WT was labeled with 114 and 116 and lepB1 mutant with 115 and 117.
ORF | Name | TMHMMa, SPb | 1st iTRAQ |
2nd iTRAQ |
Average ratio (geometric) | S.D. (log scale) | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
115:114 | 116:114 | 117:114 | 115:114 | 117:114 | 115:116 | 117:116 | |||||
PSI, plastocyanin | |||||||||||
Slr1834 | PsaA | 11 | 0.42 | 0.42 | 0.43 | 0.58 | 0.49 | 0.60 | 0.51 | 0.49 | 0.14 |
Slr1835 | PsaB | 11 | 0.44 | 0.47 | 0.44 | 0.61 | 0.54 | 0.62 | 0.55 | 0.52 | 0.13 |
Sll0819 | PsaF | 2, sec | 0.36 | 0.36 | 0.40 | 0.55 | 0.47 | 0.57 | 0.48 | 0.45 | 0.18 |
Slr1655 | PsaL | 3 | 0.36 | 0.43 | 0.46 | 0.55 | 0.48 | 0.54 | 0.47 | 0.46 | 0.14 |
Ssl0563 | PsaC | 0.48 | 0.49 | 0.27 | 0.59 | 0.45 | 0.63 | 0.47 | 0.47 | 0.25 | |
Slr0737 | PsaD | 0.27 | 0.27 | 0.33 | 0.54 | 0.46 | 0.57 | 0.48 | 0.40 | 0.30 | |
Ssr2831 | PsaE | 0.35 | 0.33 | 0.36 | 0.51 | 0.45 | 0.52 | 0.46 | 0.42 | 0.18 | |
Sll0199 | Plastocyanin | sec | 1.36 | 1.27 | 1.20 | 1.44 | 1.65 | 1.25 | 1.43 | 1.37 | 0.10 |
Cytb6f | |||||||||||
Sll1316 | Rieske protein PetC1 | 1 | 0.66 | 0.65 | 0.69 | 0.66 | 0.61 | 0.70 | 0.65 | 0.66 | 0.04 |
Sll1317 | Cyt f PetA | 1, sec | 0.59 | 0.53 | 0.55 | 0.68 | 0.60 | 0.71 | 0.62 | 0.61 | 0.10 |
Slr0342 | Cyt b6 PetB | 4 | 0.43 | 0.43 | 0.54 | 0.71 | 0.71 | 0.72 | 0.72 | 0.59 | 0.23 |
Slr0343 | Subunit IV PetD | 3 | 0.55 | 0.54 | 0.68 | 0.67 | 0.60 | 0.65 | 0.58 | 0.61 | 0.16 |
PSII | |||||||||||
Slr1311/Sll1867 | D1 | 5 | 1.03 | 1.06 | 1.28 | 1.04 | 1.07 | 1.09 | 1.09 | 1.09 | 0.07 |
Slr0927/Sll0849 | D2 | 6 | 1.33 | 1.33 | 1.18 | 1.06 | 1.01 | 1.07 | 1.02 | 1.14 | 0.11 |
Slr0906 | CP47 | 6 | 1.13 | 1.08 | 1.12 | 1.08 | 1.09 | 1.07 | 1.07 | 1.09 | 0.02 |
Sll0851 | CP43 | 7 | 1.08 | 1.08 | 1.08 | 1.07 | 1.03 | 1.09 | 1.04 | 1.07 | 0.02 |
Ssr3451 | Cyt b559 alpha subunit | 1 | 1.56 | 1.32 | 1.06 | 1.04 | 1.02 | 0.98 | 0.96 | 1.12 | 0.17 |
Ssl2598 | PsbH | 1 | 2.22 | 1.63 | 0.82 | 1.09 | 0.94 | 1.19 | 1.02 | 1.21 | 0.32 |
Sll0427 | PsbO | sec | 1.30 | 1.22 | 1.18 | 1.22 | 1.16 | 1.24 | 1.17 | 1.21 | 0.03 |
Sll1194 | PsbU | sec | 1.63 | 1.56 | 1.60 | 1.31 | 1.38 | 1.35 | 1.42 | 1.46 | 0.08 |
Sll0258 | PsbV, cyt c550 | sec | 1.28 | 1.28 | 1.32 | 1.18 | 1.08 | 1.22 | 1.11 | 1.21 | 0.07 |
Sll1638 | PsbQ | LP | 1.18 | 1.07 | 0.79 | 1.11 | 1.11 | 1.10 | 1.10 | 1.06 | 0.12 |
Sll1398 | Psb28 | 1.21 | 1.06 | 0.73 | 0.98 | 1.05 | 0.92 | 0.99 | 0.98 | 0.15 | |
Slr1645 | Psb27 | LP | 1.61 | 1.45 | 0.88 | 1.11 | 1.07 | 1.05 | 1.00 | 1.14 | 0.20 |
Phycobiliproteins | |||||||||||
Slr2067 | Allophycocyanin alpha subunit | 0.46 | 0.44 | 0.47 | 0.66 | 0.59 | 0.65 | 0.58 | 0.54 | 0.15 | |
Ssl3093 | Phycobilisome small rod linker, CpcD | 0.58 | 0.57 | 0.51 | 0.65 | 0.63 | 0.67 | 0.66 | 0.61 | 0.09 | |
Sll1577 | Phycocyanin b subunit | 0.60 | 0.56 | 0.61 | 0.74 | 0.64 | 0.75 | 0.64 | 0.65 | 0.10 | |
Sll1578 | Phycocyanin alpha subunit | 0.62 | 0.63 | 0.64 | 0.67 | 0.62 | 0.68 | 0.63 | 0.64 | 0.03 | |
Slr2051 | Phycobilisome rod-core linker, CpcG | 0.65 | 0.62 | 0.67 | 0.75 | 0.70 | 0.77 | 0.72 | 0.70 | 0.07 | |
Sll1580 | Phycobilisome Linker polypeptide | 0.68 | 0.68 | 0.74 | 0.84 | 0.84 | 0.88 | 0.88 | 0.79 | 0.11 | |
Sll0928 | Allophycocyanin B | 0.70 | 0.67 | 0.67 | 0.73 | 0.68 | 0.71 | 0.67 | 0.69 | 0.03 | |
Ssr3383 | Phycobilisome linker domain protein CpcD | 0.77 | 0.65 | 0.46 | 0.79 | 0.79 | 0.80 | 0.80 | 0.71 | 0.19 | |
Sll1579 | Phycobilisome linker polypeptide | 0.72 | 0.73 | 0.72 | 0.87 | 0.84 | 0.87 | 0.84 | 0.80 | 0.08 | |
Slr1986 | Allophycocyanin beta subunit | 0.82 | 0.72 | 0.65 | 0.72 | 0.69 | 0.73 | 0.70 | 0.72 | 0.07 | |
Sll1471 | Phycobilisome rod-core linker, CpcG | 0.72 | 0.74 | 0.83 | 0.88 | 0.90 | 0.89 | 0.91 | 0.84 | 0.09 | |
Slr1459 | Allophycocyanin, beta subunit | 0.76 | 0.76 | 0.71 | 0.74 | 0.68 | 0.75 | 0.69 | 0.73 | 0.04 | |
Slr0335 | Phycobilisome core membrane linker, ApcE | 0.89 | 0.86 | 0.89 | 0.93 | 0.92 | 0.91 | 0.90 | 0.90 | 0.02 | |
Pigment biosynthesis | |||||||||||
Slr0536 | Uroporphyrinogen decarboxylase | 0.63 | 0.60 | 0.69 | 0.68 | 0.69 | 0.73 | 0.74 | 0.68 | 0.07 | |
Sll1214 | Magnesium-protoporphyrin IX monomethyl ester cyclase | 0.33 | 0.34 | 0.35 | 0.56 | 0.48 | 0.58 | 0.50 | 0.44 | 0.23 | |
Sll1091 | Geranylgeranyl reductase, ChlP | 0.45 | 0.45 | 0.46 | 0.68 | 0.61 | 0.67 | 0.60 | 0.55 | 0.18 | |
Slr0144 | hypothetical protein 4-vinyl reductase 4VR | 0.49 | 0.53 | 0.56 | 0.63 | 0.65 | 0.61 | 0.62 | 0.58 | 0.10 | |
Redox proteins | |||||||||||
Slr5088 | short-chain dehydrogenase/ reductase SDR | 2.94 | 2.70 | 3.24 | 1.97 | 2.23 | 1.80 | 2.05 | 2.37 | 0.21 | |
Slr1379 | Cyt bd ubiquinol oxidase, CydA | 9 | 2.01 | 1.93 | 2.23 | 1.73 | 1.93 | 1.70 | 1.90 | 1.91 | 0.08 |
Sll1182 | Rieske iron-sulfur protein, PetC3 | Tat/LP | 2.20 | 2.39 | 2.36 | 1.27 | 1.17 | 1.42 | 1.31 | 1.66 | 0.30 |
Slr1239 | Pyridine nucleotide transhydrogenase, alpha subunit | 5 | 0.50 | 0.52 | 0.56 | 0.69 | 0.63 | 0.74 | 0.66 | 0.61 | 0.14 |
a Number of transmembrane helices known or predicted by the TMHMM program (http://www.cbs.dtu.dk/services/TMHMM-2.0/).
b Known or putative sec or lipoprotein signals using SignalP 3.0 (http://www.cbs.dtu.dk/services/SignalP-3.0/) and LipoP (http://www.cbs.dtu.dk/services/LipoP/), respectively.