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Radiolytic oxidation guided placement of waters near the conserved NPXXY motif of 5-HT4R. A, distribution of waters placed into the 5-HT4R model based on residue conservation. B, possible pathway of signal communication from critical residue W2726.48 through a water channel to P3097.50 for inhibition of signaling mediated through H8 and the C terminus of the 5-HT4R.
