Table II. Changes in the de-sialylated and de-sialylated/de-galactosylated glycoforms of the Hp-T3 peptide in liver disease.
HCC, cirrhotic patients with HCC (n = 10); CIR, cirrhotic patients without HCC (n = 10); DIS, combined HCC and CIR groups (n = 20); CTL, healthy controls (n = 10). The numbers represent average ± S.D. f the normalized MRM peak areas of the 204 transition. Kruskal-Wallis (CTL:CIR, CTL:HCC, and CIR:HCC) and Mann-Whitney (CTL:DIS) tests with adjustment for multiple comparisons (false discovery rate (FDR)) were used to derive p values in the group comparisons.
| Glycoform | CTL (average ± S.D.) | CIR (average ± S.D.) | HCC (average ± S.D.) | p value CTL:HCC | p value CTL:CIR | p value CIR:HCC | p value CTL:DIS | FDR CTL:DIS |
|---|---|---|---|---|---|---|---|---|
| De-sialylated Hp-T3 glycoforms | ||||||||
| A3G3 | 42.9/6.01 | 40.6/12.1 | 41.9/5.83 | 0.999 | 0.852 | 0.852 | 0.91 | 0.91 |
| A3G3F1 | 7.41/2.13 | 17.4/12.4 | 14.9/10.3 | 0.069 | 0.138 | 0.852 | 0.05 | 0.06 |
| A3G3F2 | 1.51/0.40 | 6.49/5.58 | 3.20/2.71 | 0.028 | 0.059 | 0.437 | 0.02 | 0.04 |
| A3G3F3 | 0.14/0.04 | 0.86/1.23 | 0.87/1.93 | 0.017 | 0.028 | 0.481 | 0.01 | 0.04 |
| A4G4 | 8.25/1.60 | 10.6/4.00 | 11.1/3.10 | 0.038 | 0.157 | 0.852 | 0.04 | 0.05 |
| A4G4F1 | 1.89/0.84 | 9.87/7.64 | 5.37/3.75 | 0.038 | 0.059 | 0.320 | 0.02 | 0.04 |
| A4G4F2 | 0.49/0.49 | 10.1/10.9 | 3.13/3.83 | 0.051 | 0.059 | 0.395 | 0.02 | 0.04 |
| A4G4F3 | 1.90/0.46 | 6.44/2.75 | 3.82/5.34 | 0.044 | 0.015 | 0.395 | 0.01 | 0.04 |
| A4G4F4 | 0.09/0.07 | 0.35/0.45 | 1.05/2.11 | 0.682 | 0.028 | 0.437 | 0.11 | 0.12 |
| A4G4F5 | 0.20/0.15 | 0.63/0.84 | 0.57/0.94 | 0.069 | 0.024 | 0.737 | 0.02 | 0.04 |
| De-sialylated de-galactosylated Hp-T3 glycoforms | ||||||||
| A3 | 45.4/11.2 | 52.9/14.3 | 50.3/8.17 | 0.320 | 0.227 | 0.629 | 0.19 | 0.19 |
| A3G1F1 | 6.72/2.98 | 22.5/19.0 | 18.5/14.3 | 0.060 | 0.178 | 0.852 | 0.05 | 0.06 |
| A3G1F2 | 0.14/0.06 | 0.76/0.56 | 1.06/1.94 | 0.051 | 0.002 | 0.178 | 0.00 | 0.01 |
| A3G2F2 | 0.48/0.28 | 2.83/3.77 | 8.70/14.9 | 0.044 | 0.038 | 0.682 | 0.02 | 0.02 |
| A3G2F3 | 0.09/0.05 | 0.36/0.25 | 1.49/2.79 | 0.020 | 0.017 | 0.970 | 0.01 | 0.01 |
| A4 | 11.3/4.10 | 14.9/5.67 | 14.0/4.70 | 0.157 | 0.178 | 0.682 | 0.10 | 0.11 |
| A4G1F1 | 1.88/1.48 | 12.5/11.3 | 11.0/9.47 | 0.008 | 0.060 | 0.970 | 0.01 | 0.02 |
| A4G1F2 | 0.64/0.37 | 3.46/4.12 | 11.4/23.3 | 0.009 | 0.009 | 0.629 | 0.00 | 0.01 |
| A4G2F2 | 0.39/0.25 | 0.94/1.18 | 0.70/0.94 | 0.024 | 0.069 | 0.970 | 0.02 | 0.02 |
| A4G2F3 | 0.42/2.84 | 0.82/0.65 | 1.34/1.76 | 0.069 | 0.157 | 0.852 | 0.05 | 0.06 |
| A4G3F3 | 0.20/0.23 | 0.54/0.47 | 1.51/2.47 | 0.060 | 0.033 | 1.000 | 0.02 | 0.02 |
| A4G3F4 | 0.20/0.18 | 0.63/0.62 | 3.31/7.15 | 0.009 | 0.138 | 0.481 | 0.19 | 0.19 |
| A4G4F4 | 0.25/0.08 | 0.47/0.23 | 0.55/0.49 | 0.020 | 0.008 | 0.528 | 0.05 | 0.06 |
| A4G4F5 | 0.69/0.34 | 1.52/1.14 | 1.59/1.07 | 0.006 | 0.051 | 0.629 | 0.00 | 0.01 |