Fig. 1.

An overview of the strategy combining DIA-MS with subcellular fractionation. A, Work flow of the experimental and bioinformatics procedures used for analysis of the WCL and 20KgP fractions by DIA MS. B, Equal amounts of protein from WCL and 20KgP fractions from glucose- and oleate-grown cells were separated by SDS-PAGE and visualized by Coomassie blue staining. C, Differential abundance method (Method I) compares protein abundance in a fraction from oleic acid-grown cells relative to the same fraction from glucose-grown cells. Fraction enrichment method (Method II) compares the relative abundance of identified proteins in the organelle-enriched fraction versus the crude WCL fraction within identical conditions.