FIGURE 6.

Multispecificity of the anti-AGEs mAbs. A, the immunoreactivity of anti-AGEs mAbs prepared from the Balb/c mice immunized with the DHA-modified KLH. Three hybridoma clones, BDM1, BDM2, and BDM3, producing IgM Abs specific for the AGEs were prepared from the mice. B, the immunoreactivity of the anti-AGEs mAbs prepared from MRL-lpr mice against the AGEs. Three hybridoma clones, ADL7, ADL19, and DDL17, producing the IgM Abs specific for the AGEs, and three hybridoma clones, ADL13, DDL18, and DDL20, producing IgG Abs specific for the AGEs, were prepared from the female MRL-lpr mice (20 weeks old). The IgM titers were determined by ELISA using dsDNA and native and DHA-modified proteins as the absorbed antigens.