FIGURE 8.

Involvement of electronegative potential of antigens in the recognition by the anti-AGEs mAbs. A, cross-reactivity of the sera from the MRL-MpJ and MRL-lpr mice with native BSA (open bar) and acetylated BSA (AcBSA, closed bar). B, cross-reactivity of the anti-AGEs IgM BDM1 established from the Balb/c mice immunized with the DHA-modified KLH with acetylated BSA. The antibody titers were determined by ELISA using the native BSA and DHA-modified and acetylated BSA as the absorbed antigens. C, flow cytometric analysis of antigen-antibody interaction. The solid phase paramagnetic beads coupled to the antigens, BSA (black line), AGEs (blue line), and acetylated BSA (red line), were used as the probes. The control mouse IgM (left panel) and representative anti-AGEs IgM BDM1 (right panel) were tested for binding to the protein-coupled beads. The number of binding events is plotted against fluorescence intensity.