FIGURE 3.

Effects of GAr spacing and composition on production of intermediates. A, GST-I27-GAr₁₀-GFP-ssrA substrate with different length spacers between I27 and the GAr₁₀ motif was degraded by ClpXP. The lengths of the spacers were 0, 11, or 22 amino acids. The position of full-length substrate (solid arrows) or degradation intermediate (dashed arrows) is indicated. B, the production of intermediates from substrates I27-test-GFP-ssrA with test sequences Gly₁₀ and Ala₁₀ were compared with GAr₁₀ and with a 10-mer control₁₀ of diverse composition (sequence shown). Data were generated and analyzed as in Fig. 2. Error bars, S.D.