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Percentage of intermediate production by single residue X substitution in Ala4-X-Ala5
The substrate consisted of GST-I27–10-mer-GFP-ssrA. Test 10-mers are listed in descending order of potency for producing intermediates.
| Test sequence (10-mer) | Percentage of intermediate |
|---|---|
| % | |
| A4GA5 | 56 ± 7 |
| A10 | 56 ± 6 |
| A4VA5 | 51 ± 7 |
| A4YA5 | 49 ± 6 |
| A4KA5 | 48 ± 7 |
| A4HA5 | 47 ± 5 |
| A4SA5 | 44 ± 5 |
| A4DA5 | 38 ± 4 |
| A4FA5 | 35 ± 4 |
| A4EA5 | 32 ± 4 |
| A4WA5 | 32 ± 5 |
| A4RA5 | 22 ± 4 |
