FIGURE 1.

Cleavage of E-cadherin by GAS culture supernatant. Culture supernatants from several GAS clinical isolates were incubated with the recombinant extracellular domain of E-cadherin for 6 h at 37 °C. Sample proteins were separated by SDS-PAGE under a reducing condition and subjected to immunoblotting using an antibody against the extracellular domain of E-cadherin and the horseradish peroxidase-conjugated secondary antibody. The signals were developed with the peroxidase substrate tetramethylbenzidine. GAS strains recovered from invasive disease are underlined.