FIGURE 5.

SpeB is involved in the process of GAS translocation. Caco-2 cells were grown on Millicell filters and then infected with GAS strains at an MOI of 10 for 2 h. After removing nonadhered bacteria, the ability of the GAS strains to translocate across epithelial cells was assessed by examining medium samples obtained from the lower chambers at the indicated time of infection. Shown are the results of translocation rates of strain NIH35 (A) and clinical isolates (B). Three experiments were performed, and the data are presented as the means ± S.D. of six samples from representative findings obtained in three independent experiments. *, p < 0.01.