FIGURE 7.
Hha augments H-NS DNA binding in vitro. A, electrophoretic mobility shift assays performed with a 300-bp PCR product from the hilA promoter region. No protein was added to the samples marked with minus signs. The plus signs signify 300 nm H-NS in the binding reaction. The addition of HhaWT to the samples indicated at concentrations of 300, 600, 900, and 1200 nm resulted in increased shifting of the hilA fragment. When HhaWT is combined with the hilA fragment in the absence of H-NS, no shifting is observed. B, EMSAs performed with HhaR14R17A and HhaD48N also result in improved shifting of the hilA fragment. The same protein concentrations from A were applied.