FIGURE 2.

d16-base sphingolipids comprise a significant part of the cardiac sphingolipidome. d16:0-base sphingolipids make up approximately one-third of total dihydrosphingosine (A) and total dihydroceramide (B), whereas d16:1-ceramide (C) and d16:1-sphingosine (D) comprise a smaller proportion of total ceramide and sphingosine, respectively. Hearts were harvested from six 16-week-old adult male C57Bl6/J mice, homogenized, and subjected to LC-MS analysis as described under “Materials and Methods.” Results were normalized to protein content, and relative base composition was calculated for each sphingolipid species in each mouse. Results were aggregated and are presented as mean ± S.E. (error bars). *, p < 0.05; n.s., not significant (p ≥ 0.05).