DAI expression is notably increased in PBMCs of SLE patients.
A, real-time PCR analysis of DAI mRNA levels in PBMCs from healthy normal controls (n = 30), patients with acute bacterial pneumonia (n = 25), tuberculosis (n = 20), asthma (n = 26), type-I diabetes (n = 25), and SLE (n = 30). B, Western blot analysis of DAI protein levels in PBMCs from SLE patients (S) and relative healthy normal controls (N). Data are representative of results obtained in three independent experiments, n = 16. C, graphical representations of band intensities in B. Expression of DAI was normalized to GAPDH expression. D, levels of IFN-α in serum of SLE patients and healthy normal controls were determined by ELISA. Data are mean ± S.E. of three independent experiments.