FIGURE 8.

MATIIα and SETDB1 regulate COX-2 gene expression. A, effects of siRNA on the expression levels of SETDB1 and MATIIα in iMEFs. B, qPCR analysis of COX-2 gene expression. The averages of three independent experiments with standard deviations are shown. p values (Student's t test) for differences between the control and SETDB1 KD are indicated. C and D, ChIP assays were performed using an anti-SETDB1 and Suv39h1 antibody with chromatin from iMEFs. The relative levels of enrichment of the indicated genomic DNA regions are shown with S.D. p values (Student's t test) for differences between the pairs of antibodies are indicated. E, a model for the function of MATII-SETDB1 in the regulation of COX-2 gene expression. IB, immunoblot.