FIGURE 2.

rBbKIm induces apoptosis and cell arrest in prostate cancer lineages after 48 h of treatment. The cells were exposed to 50 and 100 μm of rBbKIm and SbTI for 24 and 48 h. A–D, subsequently, the cell cycle was evaluated using the PI label, G₀/G₁ (black columns), S (white columns), and G₂/M (gray columns). E–H, cell death was assessed by flow cytometry using the annexin V-FITC and PI label, annexin V⁻PI⁻ (black columns), annexin V⁺PI⁻ (light gray columns), annexin V⁺PI⁺ (dark gray columns), and annexin V⁻PI⁺ (white columns). A and B, rBbKIm promotes cell cycle arrest after 48 h of treatment in PC3 cells (B) but not in DU145 cells (A). C and D, SbTI had no effect on cell cycle in both cells. E and F, low levels of cell death at 24 h of rBbKIm treatment in DU145 (E) and PC3 (F) cells was observed; however, after 48 h, ∼50% of the cells died by apoptosis (annexin V⁺PI⁻). G and H, SbTI did not induced DU145 (G) and PC3 (H) apoptosis. The experiment is representative of two independent experiments. The values are expressed as the means ± standard deviation. Significant differences versus controls are presented (ANOVA; *, p < 0.05).