FIGURE 3.

The effect of ascorbate on 5-hmC is blocked by phloretin. A, immunostaining shows that phloretin (100 μm) does not change 5-hmC signal in ascorbate-free MEFs but does block the induction of 5-hmC by ascorbate (10 μm) treatment for 24 h. B, a representative dot-blot shows that phloretin (100 μm) inhibits the effects of ascorbate (10 μm) on 5-hmC content. C, semiquantitative analysis of the dot-blot indicates that phloretin inhibits the induction of 5-hmC by ascorbate (*, p < 0.05 assessed by Student's t test; data are represented as mean ± S.E.).