Primary cilia on Hap1-deficient cells have normal lengths and can properly undergo cilia disassembly under growth factor stimulation conditions.
A, the percentage of ciliated MEFs from Hap1+/+ and Hap1−/− mice show similar declining patterns during serum stimulation. MEFs isolated from Hap1+/+ (n = 4) and Hap1−/− (n = 6) embryos were cultured in serum starved conditions for 24 h prior to serum stimulation. After various incubation times with serum, MEFs were fixed for Arl13b cilium immunolabeling (>100 cells were counted from each coverslip). No significant differences were found between Hap1+/+ and Hap1−/− MEFs. Error bars represent the S.E. B, in Hap1−/− neurons, primary cilia lengths were similar to wild type cells. Hypothalamic cells from Hap1+/+ (n = 3) and Hap1−/− (n = 3) embryos were immunolabeled with the ciliary marker, AC3, and cilia lengths were measured using AxioVision software. Range of cilia lengths: Hap1+/+, 1.29–8.22 μm and Hap1−/−, 1.10–8.34 μm. Error bars represent the S.E.