FIGURE 1.
The Del-L955 deletion mutation hyperpolarizes activation of NaV1.7 channel. A, schematic of the human NaV1.7 channel topology showing residue Leu955. All of the rescue mutations introduced in this study are listed in below. B and C, representative traces of current families recorded from HEK293 cells expressing WT (B) and Del-L955 (C) channel. D, Del-L955 mutant channel studied in parallel with WT channel in HEK293 cells using a voltage dependence of activation protocol. Del-L955: −50 ± 1.6 mV, n = 11; NaV1.7 WT: −23.8 ± 1.4 mV, n = 7, p < 0.001. Stimulus protocol for activation is also shown as an inset.