FIGURE 2.
Radial displacement of the activation gate (Phe960) in Del-L955 channel. A, structural model of WT NaV1.7 channel shows four residues, one from each domain (Tyr405 of DI, Phe960 of DII, Phe1449 of DIII, and Phe1752 of DIV), forms the activation gate. Phe960 is colored red, and Ser961 is colored yellow; both of them are circled for highlighting. B, in the Del-L955 structural model, when Leu955 is deleted, the orientation of Phe960 rotates radially toward the S6 of domain III. The Leu955 deletion also rotates Ser961 to the previous location of Phe960 so that the side chain of Ser961 now points to the pore, facing the other three activation gate residues. Phe960 of Del-L955 channel is shown in red, and Ser961 is shown in yellow. Both are circled for highlighting. C, intramembrane view shows four S6 helices of the Del-L955 mutant channel structural model. All other structural elements are omitted for clarity. D, extracellular view shows the four S6 helices of the Del-L955 mutant channel structural model.