FIGURE 6.

A, purified NemR variants (2 μg), with and without incubation with a 1:1 molar ratio of N-chlorotaurine (NCT), were visualized by nonreducing or reducing SDS-PAGE, as indicated. B, E. coli BL21 expressing His-tagged NemR variants were grown to mid-log phase, induced with 10 μm isopropyl 1-thio-β-d-galactopyranoside for 30 min, then treated with 1 mm HOCl and harvested at indicated time points. Reduced cysteine thiols were irreversibly alkylated with iodoacetamide, and NemR was visualized by Western blotting using nonreducing SDS-PAGE.