FIGURE 7.

Cellulose synthesis by recombinant enzyme. Cellohexaose was incubated with recombinant enzyme (0.5 μg) in 50 mm sodium phosphate buffer (pH 6.0) in the presence or absence of glucose at 40 °C. The mixtures were centrifuged and photographed (A). Insoluble material was dissolved in 72% (w/v) H₂SO₄, and the sugar content was determined using anthrone in H₂SO₄ (B). Water-insoluble material was observed by electron microscopy with negative staining (C) and the lamellar structure was subjected to electron diffraction analysis (D). Molecular mass of the sodium ion adducts of the water-insoluble substance was determined by MALDI-TOF MS (E).